PCR detection of hemolysin (vhh) gene in Vibrio harveyi
نویسندگان
چکیده
منابع مشابه
Overexpression, purification, characterization, and pathogenicity of Vibrio harveyi hemolysin VHH.
Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity, cytotoxicity, pathogenicity to flounder, and the signal peptide.
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The virulence factors of Vibrio harveyi, the causative agent of luminous vibriosis, are not completely understood. We investigated the correlations between shrimp mortality, hemolysis, the presence of a hemolysin gene (vhh), and a gene involved in the type III secretion system (the Vibrio calcium response gene vcrD). V harveyi HY01 was isolated from a shrimp that died from vibriosis, and 36 oth...
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Vibrio harveyi VIB 645, which is very pathogenic towards salmonids and produces extracellular product with a high titer of hemolytic activity towards fish erythrocytes, was found to contain two closely related hemolysin genes (designated vhhA and vhhB), whereas the majority of strains examined (11 of 13) carried only a single hemolysin gene. Both genes from VIB 645 were cloned and sequenced. Th...
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ژورنال
عنوان ژورنال: The Journal of General and Applied Microbiology
سال: 2004
ISSN: 0022-1260,1349-8037
DOI: 10.2323/jgam.50.137